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KMID : 0352720070310040196
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Journal of Ginseng Research
2007 Volume.31 No. 4 p.196 ~ p.202
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Effects of Korean red ginseng and herb extracts mixture (KTNG0345) on procollagen biosynthesis and matrix metalloproteinase-1 activity in human dermal fibroblast
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So Seung-Ho
Lee Seong-Kye
Hwang Eui-Il
Koo Bon-Suk
Han Gyeong-Ho
Kim Na-Mi
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Abstract
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Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-Rb₁, torilin and loganin were identified at 10.85 ㎎/g, 0.128 ㎎/g and 3.92 ㎎/g respectively.
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KEYWORD
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human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1), SOD activity, Korean red ginseng
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